Supporting Informations Human serum biomarker detection based on cascade signal amplification strategy by DNA molecule machine
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چکیده
Reagents. Thioglycolic acid (TGA), Bovine serum albumin (BSA), imidazole, N-(3dimethylaminopropyl)-N’-ethyl-carbodiimide hydrochloride (EDC), Nhydroxysuccinimide (NHS), (+)-Biotin N-hydroxysuccinimide were purchased from Sigma-Aldrich. EDC and NHS were dissolved in water immediately before use. Human prostate-specific antigen (PSA), two mouse antihuman total PSA monoclonal antibodies (primary capture antibody, Ab1 and biotin linked secondary detection antibody, Biotin-Ab2) were received from Shanghai Linc-Bio Science Co., Ltd. New England Biolabs (USA) provided Nb.BtSI and NEBuffer, while Klenow exo minus was bought from Thermo Fisher Scientific (USA). Streptavidin was from AMRESCO All other reagents were of analytical reagent grade. DNA oligonucleotides labeled with Cy5 and BHQ, and a dNTP mix stock solution were obtained from TaKaRa Biotechnology (Dalian) Co., Ltd. The sequences of employed oligonucleotides are as Table 1: Table S1. The oligonucleotide sequences used in this study
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تاریخ انتشار 2015